mlpA

168

metallopeptidase, involved in regulation of protease gene expression

locus: BSU_16710

Molecular weight: 45.80 kDa

pI: 5.28

Protein length: 409 aa

Gene length: 1230 bp

function: control of proteolytic activity

product: specific processing protease

essential: no

synonyms: mlpA, ymxG

List of homologs in different organisms, belongs to COG0612 (Galperin et al., 2021)

Gene

Coordinates: 1,742,617  1,743,846

Phenotypes of a mutant

• fivefold increased levels of proteolytic activity in their growth medium [Pubmed|10666719]

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The protein

Protein family

• [wiki|Peptidase M16 family] (according to UniProt)

Structure

• [PDB|3HDI] (from B. halodurans, 64% identity) [pubmed|19913481]
• [AF|Q04805]

Expression and Regulation

Operons

• genes: [gene|2AD0F2C0B0957A9E77EF9771D010ED77459B8CC0|mlpA]

  description: [Pubmed|8098035]

Biological materials

Mutant

• MGNA-A057 (ymxG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/57 NBRP B. subtilis, Japan]
• deletion mutant,  available in [wiki|van Dijl] lab
• BKE16710 ([gene|2AD0F2C0B0957A9E77EF9771D010ED77459B8CC0|mlpA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAATTTGTTCCTCCTATTC,  downstream forward: _UP4_TAAAAAGGAAAGCCTGCCCC
• BKK16710 ([gene|2AD0F2C0B0957A9E77EF9771D010ED77459B8CC0|mlpA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAATTTGTTCCTCCTATTC,  downstream forward: _UP4_TAAAAAGGAAAGCCTGCCCC

References

Reviews

• 10974125,34410368

Original Publications

• 10666719,10974125,8098035,19913481

Labs working on this gene/protein

• [wiki|Jan Maarten van Dijl], Groningen, Netherlands