mleN

mleN
168

malate-H+/Na+-lactate antiporter, uptake of D-asparagine

locus
BSU_23560
Molecular weight
50.05 kDa
pI
7.25
Protein length
Gene length
function
malate uptake
product
malate-H+/Na+-lactate antiporter
essential
no
synonyms
mleN, yqkI

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1757 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,452,800  2,454,206
Phenotypes of a mutant
resistant to otherwise toxic D-asparagine [pubmed|38193659]
abrupt arrest in the mid-logarithmic phase of growth on malate when low concentrations of protonophore were present [Pubmed|10903309]
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The protein
Catalyzed reaction/ biological activity
uptake of D-asparagine [pubmed|38193659]
Protein family
NhaC Na(+)/H(+) (TC 2.A.35) antiporter family (with [protein|A7F1B4C0D32E18773170BFBEEB94E5EF55C7DB97|nhaC], according to UniProt)
Structure
[AF|P54571]
Paralogous protein(s)
[protein|A7F1B4C0D32E18773170BFBEEB94E5EF55C7DB97|nhaC]
[wiki|Localization]
membrane associated [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]-[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]
description
[Pubmed|22383849]
regulation
''[protein|search|ansA]'': expressed in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]
regulatory mechanism
[protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: repression, [Pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|22900538], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1711029], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]→[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]

2024-03-21 23:44:28

ghost

52

2adb475a1d359d8484e1d13c3462a134569ab20a

12E51CE78001847EEC34BF1A5209896CF1E02F0D

genes
[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]-[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]-[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]
description
[Pubmed|22383849]
regulation
[protein|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]: expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression
expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
[protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: transcription repression, [pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]
Open in new tab

[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]→[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]

2024-03-23 21:02:52

Jstuelk

63

6E6CC6ADE2F20EBE93C9F1ED4C2EF4E87AF126B1

D7F0E184034763E326DE2841E35068D2FD73DD5D

Biological materials
Mutant
GP1460 (Δ[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]::''kan''), available in  [wiki|Jörg Stülke]'s lab [pubmed|38193659]
GP4165 (Δ[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]::''kan''), available in  [wiki|Jörg Stülke]'s lab
MGNA-C411 (yqkI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2409 NBRP B. subtilis, Japan]
GP1460 (''mleN''::''spc''), available in [wiki|Jörg Stülke]'s lab
BKE23560 ([gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAAAGAAGTCCCCCTTCAT,  downstream forward: _UP4_GGCTAATAGGGGAGGATCAC
BKK23560 ([gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAAAGAAGTCCCCCTTCAT,  downstream forward: _UP4_GGCTAATAGGGGAGGATCAC
lacZ fusion
pGP388 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab [pubmed|38193659]
References
36815589,18763711,10903309,22900538,22383849,1711029,11914346,38193659

49DF23B8E19658DB32835B6D23DD21E4BF481B20

Page visits: 4599

Time of last update: 2024-03-28 16:52:53

Author of last update: Jstuelk