Difference between revisions of "MleN"

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(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutant:'''GP1460 (''mleN''::''spc''), available in [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 08:28, 2 November 2012

  • Description: malate-H+/Na+-lactate antiporter

Gene name mleN
Synonyms yqkI
Essential no
Product malate-H+/Na+-lactate antiporter
Function malate uptake
Gene expression levels in SubtiExpress: mleN
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 50 kDa, 7.249
Gene length, protein length 1404 bp, 468 aa
Immediate neighbours mleA, ansB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MleN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MleN expression.png



















Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

AnsR regulon, CcpA regulon

The gene

Basic information

  • Locus tag: BSU23560

Phenotypes of a mutant

abrupt arrest in the mid-logarithmic phase of growth on malate when low concentrations of protonophore were present PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: nhaC Na(+)/H(+) antiporter family (according to Swiss-Prot)
  • Paralogous protein(s): NhaC

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:GP1460 (mleN::spc), available in Stülke lab
  • Expression vector:
  • lacZ fusion: pGP388 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References