mleA

168

malic enzyme

locus: BSU_23550

Molecular weight: 45.73 kDa

pI: 4.89

Protein length: 439 aa

Gene length: 1320 bp

function: malate utilization

product: NAD-dependent malate dehydrogenase

essential: no

ec: 1.1.1.38

synonyms: mleA, yqkJ

List of homologs in different organisms, belongs to COG0281 (Galperin et al., 2021)

Gene

Coordinates: 2,451,463  2,452,782

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The protein

Catalyzed reaction/ biological activity

• (S)-malate + NAD⁺ --> CO₂ + NADH + pyruvate (according to UniProt)
• H⁺ + oxaloacetate --> CO₂ + pyruvate (according to UniProt)

Protein family

• [wiki|malic enzymes family] (according to UniProt)

[wiki|Domains]

• [wiki|ACT domain] (aa 9-84) (according to UniProt)

Structure

• [PDB|2A9F] (from Streptococcus pneumoniae, 42% identity from aa 90 - 435)
• [AF|P54572]

Paralogous protein(s)

• [protein|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ], [protein|BBBCD5F56779895189E21076AF165B901F654534|malS]

Biological materials

Mutant

• MGNA-C412 (yqkJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2410 NBRP B. subtilis, Japan]
• GP1136 (cat) available in [wiki|Jörg Stülke]'s lab
• BKE23550 ([gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TATCATTCAGTGATCCTCCC,  downstream forward: _UP4_TAAATGTAGCTAAACAGCAC
• BKK23550 ([gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TATCATTCAGTGATCCTCCC,  downstream forward: _UP4_TAAATGTAGCTAAACAGCAC

References

• 16788182,11914346,22383849,23136871