MifM

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  • Description: ribosome-nascent chain sensor of membrane protein biogenesis

Gene name mifM
Synonyms yqzJ
Essential no
Product sensor of SpoIIIJ activity
Function control of yidC2 translation
Gene expression levels in SubtiExpress: mifM
Interactions involving this protein in SubtInteract: MifM
MW, pI 11 kDa, 5.829
Gene length, protein length 285 bp, 95 aa
Immediate neighbours polY1, yidC2
Sequences Protein DNA Advanced_DNA
Genetic context
YqzJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MifM expression.png















Categories containing this gene/protein

translation, membrane proteins

This gene is a member of the following regulons

The MifM regulon: yidC2

The gene

Basic information

  • Locus tag: BSU23880

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the yidC2 mRNA PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • N-terminal transmembrane domain PubMed
    • the C-terminal domain interacts with the ribosome and arrests the own translation elongation PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • An antisense RNA is predicted for mifM PubMed
    • MifM has intrinsic sequences to arrest ribosome function, the arrest is influenced by the cotranslational engagement in the protein localization reaction PubMed
    • elongation proceeds when SpoIIIJ integrates the nascent chain of MifM into the membrane PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Shinobu Chiba, Koreaki Ito
Multisite ribosomal stalling: a unique mode of regulatory nascent chain action revealed for MifM.
Mol Cell: 2012, 47(6);863-72
[PubMed:22864117] [WorldCat.org] [DOI] (I p)

Gene-Wei Li, Eugene Oh, Jonathan S Weissman
The anti-Shine-Dalgarno sequence drives translational pausing and codon choice in bacteria.
Nature: 2012, 484(7395);538-41
[PubMed:22456704] [WorldCat.org] [DOI] (I e)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Shinobu Chiba, Takashi Kanamori, Takuya Ueda, Yoshinori Akiyama, Kit Pogliano, Koreaki Ito
Recruitment of a species-specific translational arrest module to monitor different cellular processes.
Proc Natl Acad Sci U S A: 2011, 108(15);6073-8
[PubMed:21383133] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Shinobu Chiba, Anne Lamsa, Kit Pogliano
A ribosome-nascent chain sensor of membrane protein biogenesis in Bacillus subtilis.
EMBO J: 2009, 28(22);3461-75
[PubMed:19779460] [WorldCat.org] [DOI] (I p)