MifM

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  • Description: ribosome-nascent chain sensor of membrane protein biogenesis

Gene name mifM
Synonyms yqzJ
Essential no
Product sensor of SpoIIIJ activity
Function control of yqjG translation
MW, pI 11 kDa, 5.829
Gene length, protein length 285 bp, 95 aa
Immediate neighbours polY1, yqjG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YqzJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MifM expression.png
























Categories containing this gene/protein

translation, membrane proteins

This gene is a member of the following regulons

The MifM regulon: yqjG

The gene

Basic information

  • Locus tag: BSU23880

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the yqjG mRNA PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
    • the C-terminus of MifM interacts with the ribosomal polypeptide exit tunnel PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Gene-Wei Li, Eugene Oh, Jonathan S Weissman
The anti-Shine-Dalgarno sequence drives translational pausing and codon choice in bacteria.
Nature: 2012, 484(7395);538-41
[PubMed:22456704] [WorldCat.org] [DOI] (I e)

Shinobu Chiba, Takashi Kanamori, Takuya Ueda, Yoshinori Akiyama, Kit Pogliano, Koreaki Ito
Recruitment of a species-specific translational arrest module to monitor different cellular processes.
Proc Natl Acad Sci U S A: 2011, 108(15);6073-8
[PubMed:21383133] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Shinobu Chiba, Anne Lamsa, Kit Pogliano
A ribosome-nascent chain sensor of membrane protein biogenesis in Bacillus subtilis.
EMBO J: 2009, 28(22);3461-75
[PubMed:19779460] [WorldCat.org] [DOI] (I p)