MiaA

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  • Description: tRNA isopentenylpyrophosphate transferase

Gene name miaA
Synonyms
Essential no
Product tRNA isopentenylpyrophosphate transferase
Function tRNA modification
Gene expression levels in SubtiExpress: miaA
MW, pI 35 kDa, 6.002
Gene length, protein length 942 bp, 314 aa
Immediate neighbours ymaF, hfq
Sequences Protein DNA Advanced_DNA
Genetic context
MiaA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MiaA expression.png
























Categories containing this gene/protein

translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU17330

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed


The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine (according to Swiss-Prot)
  • Protein family: IPP transferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

The corresponding gene/ protein in other bacteria

J Zhao, H E Leung, M E Winkler
The miaA mutator phenotype of Escherichia coli K-12 requires recombination functions.
J Bacteriol: 2001, 183(5);1796-800
[PubMed:11160115] [WorldCat.org] [DOI] (P p)

J M Durand, G R Björk, A Kuwae, M Yoshikawa, C Sasakawa
The modified nucleoside 2-methylthio-N6-isopentenyladenosine in tRNA of Shigella flexneri is required for expression of virulence genes.
J Bacteriol: 1997, 179(18);5777-82
[PubMed:9294434] [WorldCat.org] [DOI] (P p)

J Caillet, L Droogmans
Molecular cloning of the Escherichia coli miaA gene involved in the formation of delta 2-isopentenyl adenosine in tRNA.
J Bacteriol: 1988, 170(9);4147-52
[PubMed:3045085] [WorldCat.org] [DOI] (P p)