miaA

168

tRNA isopentenylpyrophosphate transferase

locus: BSU_17330

Molecular weight: 35.52 kDa

pI: 6

Protein length: 314 aa

Gene length: 945 bp

function: tRNA modification

product: tRNA isopentenylpyrophosphate transferase

essential: no

ec: 2.5.1.8

synonyms: miaA

List of homologs in different organisms, belongs to COG0324 (Galperin et al., 2021)

Gene

Coordinates: 1,866,389  1,867,333

Phenotypes of a mutant

• inactivation of ''[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]'' reduces sporulation efficiency to 16% that of wild type cells [Pubmed|26735940]

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The protein

Catalyzed reaction/ biological activity

• adenosine³⁷ in tRNA + dimethylallyl diphosphate --> diphosphate + N⁶-dimethylallyladenosine³⁷ in tRNA (according to UniProt)

Protein family

• IPP transferase family (single member, according to UniProt)

Structure

• [PDB|2QGN] (from ''Bacillus halodurans'', 57% identity)
• [AF|O31795]

Biological materials

Mutant

• BKE17330 ([gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATATGTATCACTCTCTTT,  downstream forward: _UP4_TAATCGAAACTGTATGATAT
• BKK17330 ([gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATATGTATCACTCTCTTT,  downstream forward: _UP4_TAATCGAAACTGTATGATAT

References

The corresponding gene/ protein in other bacteria

• 11160115,3045085,9294434,26735940,35212379