MgsA

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  • Description: methylglyoxal synthase

Gene name mgsA
Synonyms ypjF, jojF
Essential no
Product methylglyoxal synthase
Function bypass of glycolysis
Metabolic function and regulation of this protein in SubtiPathways:
Lys, Thr, Central C-metabolism
MW, pI 14 kDa, 4.919
Gene length, protein length 411 bp, 137 aa
Immediate neighbours bshB1, dapB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MgsA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU22480

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Glycerone phosphate = methylglyoxal + phosphate (according to Swiss-Prot)
  • Protein family: RNase Z family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1B93 (from Escherichia coli, 50% identity, 73% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • Antibody:

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Your additional remarks

References

Structure

D Saadat, D H Harrison
The crystal structure of methylglyoxal synthase from Escherichia coli.
Structure: 1999, 7(3);309-17
[PubMed:10368300] [WorldCat.org] [DOI] (P p)

Operon and expression

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)