Difference between revisions of "MgsA"

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Revision as of 13:44, 16 May 2013

  • Description: methylglyoxal synthase

Gene name mgsA
Synonyms ypjF, jojF
Essential no
Product methylglyoxal synthase
Function bypass of glycolysis
Gene expression levels in SubtiExpress: mgsA
Interactions involving this protein in SubtInteract: MgsA
Metabolic function and regulation of this protein in SubtiPathways:
Lys, Thr, Central C-metabolism
MW, pI 14 kDa, 4.919
Gene length, protein length 411 bp, 137 aa
Immediate neighbours bshB1, dapB
Sequences Protein DNA DNA_with_flanks
Genetic context
MgsA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MgsA expression.png




























Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22480

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Glycerone phosphate = methylglyoxal + phosphate (according to Swiss-Prot)
  • Protein family: RNase Z family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • non-phosphorylated Crh interacts with MgsA to inhibit its activity PubMed


Database entries

  • Structure: 1B93 (from Escherichia coli, 50% identity, 73% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • GP67 (mgsA::tet), available in Stülke lab
    • GP84 (mgsA::pX2(cat)), available in Stülke lab
  • Expression vector:
    • pGP1301 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Stülke lab
    • pGP1180 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • pGP1181 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Stülke lab
    • pGP2207 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP1459), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • FLAG-tag construct: GP86 (spc, based on pGP1331), available in the Stülke lab
  • Antibody:

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Your additional remarks

References

Rachit Jain, Yajun Yan
Dehydratase mediated 1-propanol production in metabolically engineered Escherichia coli.
Microb Cell Fact: 2011, 10;97
[PubMed:22074179] [WorldCat.org] [DOI] (I e)

Jens J Landmann, Ricarda A Busse, Jan-Hendrik Latz, Kalpana D Singh, Jörg Stülke, Boris Görke
Crh, the paralogue of the phosphocarrier protein HPr, controls the methylglyoxal bypass of glycolysis in Bacillus subtilis.
Mol Microbiol: 2011, 82(3);770-87
[PubMed:21992469] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)

D Saadat, D H Harrison
The crystal structure of methylglyoxal synthase from Escherichia coli.
Structure: 1999, 7(3);309-17
[PubMed:10368300] [WorldCat.org] [DOI] (P p)