Difference between revisions of "MgsA"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22480 mgsA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22480 mgsA]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MgsA MgsA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MgsA MgsA]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/lys_threo.html Lys, Thr], [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/lys_threo.html Lys, Thr], [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''

Revision as of 08:42, 12 November 2013

  • Description: methylglyoxal synthase

Gene name mgsA
Synonyms ypjF, jojF
Essential no
Product methylglyoxal synthase
Function bypass of glycolysis
Gene expression levels in SubtiExpress: mgsA
Interactions involving this protein in SubtInteract: MgsA
Metabolic function and regulation of this protein in SubtiPathways:
Lys, Thr, Central C-metabolism
MW, pI 14 kDa, 4.919
Gene length, protein length 411 bp, 137 aa
Immediate neighbours bshB1, dapB
Sequences Protein DNA DNA_with_flanks
Genetic context
MgsA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MgsA expression.png















Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

Spx regulon


The gene

Basic information

  • Locus tag: BSU22480

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Glycerone phosphate = methylglyoxal + phosphate (according to Swiss-Prot)
  • Protein family: RNase Z family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • non-phosphorylated Crh interacts with MgsA to inhibit its activity PubMed


Database entries

  • Structure: 1B93 (from Escherichia coli, 50% identity, 73% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
    • induced by diamide stess (thiol depletion) (Spx) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • GP67 (mgsA::tet), available in Stülke lab
    • GP84 (mgsA::pX2(cat)), available in Stülke lab
  • Expression vector:
    • pGP1301 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Stülke lab
    • pGP1180 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • pGP1181 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Stülke lab
    • pGP2207 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP1459), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • FLAG-tag construct: GP86 (spc, based on pGP1331), available in the Stülke lab
  • Antibody:

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Your additional remarks

References