mgsA

mgsA
168

methylglyoxal synthase

locus
BSU_22480
Molecular weight
14.99 kDa
pI
4.92
Protein length
Gene length
function
bypass of glycolysis
product
methylglyoxal synthase
essential
no
ec
4.2.3.3
synonyms
mgsA, ypjF, jojF

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1803 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,358,911  2,359,324
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The protein
Catalyzed reaction/ biological activity
dihydroxyacetone phosphate --> methylglyoxal + phosphate (according to UniProt)
Protein family
methylglyoxal synthase family (single member, according to UniProt)
[wiki|Domains]
MGS-like domain (aa 1-137) (according to UniProt)
Structure
[PDB|6F2C] [pubmed|29514981]
[AF|P42980]
Effectors of protein activity
non-phosphorylated [protein|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh] interacts with [protein|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA] to inhibit its activity [Pubmed|29514981,21992469]
Expression and Regulation
Operons
genes
[gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]-[gene|5AB90032031653A913E15B26B3E1AE246E95AABF|dapB]-[gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]-[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]-[gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]-[gene|4C4FA59FF5D100E941EF470C0C54E85681ADC74F|cca]-[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]
description
[Pubmed|20308541]
regulation
induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|23894131], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23894131], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]→[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]

2024-01-29 08:50:51

ghost

48

53bf67b167bd77fe013a5e22a2f6ddcbf0c547e2

73042872A92944881DD9D94DF293F1857E4735FB

Biological materials
Mutant
MGNA-A440 ([gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/440 NBRP B. subtilis, Japan]
GP67 ([gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]::''tet'') [Pubmed|21992469], available in [wiki|Jörg Stülke]'s lab
GP84 (''mgsA''::''pX2''(''cat'')), available in [wiki|Jörg Stülke]'s lab
BKE22480 ([gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22480 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC,  downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA
BKK22480 ([gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22480 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC,  downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA
Expression vectors
pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab
pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP382]), available in [wiki|Jörg Stülke]'s lab
pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP1459]), available in [wiki|Jörg Stülke]'s lab
pGP2505 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab [Pubmed|21992469]
FLAG-tag construct
GP86 (spc, based on [wiki|pGP1331]) [Pubmed|21992469], available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Boris Görke], University of Vienna, Austria
[wiki|Jörg Stülke], University of Göttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage]
References
10368300,21992469,20308541,22074179,23894131,28807600,29514981

341F198B2BD4261B20C00181D084A7C5E33E39B3

Page visits: 4441

Time of last update: 2024-03-28 01:44:26

Author of last update: Melvin.boenninger