Difference between revisions of "MgsA"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[Spx regulon]]}}
  
  
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mgsA_2358911_2359324_-1 mgsA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mgsA_2358911_2359324_-1 mgsA] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:''' [[SigA]]  {{PubMed|23894131}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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** induced by diamide stess (thiol depletion) ([[Spx]]) {{PubMed|23894131}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
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** [[Spx]]: transcription activation {{PubMed|23894131}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
<pubmed> 10368300 21992469 20308541 22074179 </pubmed>  
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<pubmed> 10368300 21992469 20308541 22074179 23894131</pubmed>  
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:14, 1 August 2013

  • Description: methylglyoxal synthase

Gene name mgsA
Synonyms ypjF, jojF
Essential no
Product methylglyoxal synthase
Function bypass of glycolysis
Gene expression levels in SubtiExpress: mgsA
Interactions involving this protein in SubtInteract: MgsA
Metabolic function and regulation of this protein in SubtiPathways:
Lys, Thr, Central C-metabolism
MW, pI 14 kDa, 4.919
Gene length, protein length 411 bp, 137 aa
Immediate neighbours bshB1, dapB
Sequences Protein DNA DNA_with_flanks
Genetic context
MgsA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MgsA expression.png















Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

Spx regulon


The gene

Basic information

  • Locus tag: BSU22480

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Glycerone phosphate = methylglyoxal + phosphate (according to Swiss-Prot)
  • Protein family: RNase Z family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • non-phosphorylated Crh interacts with MgsA to inhibit its activity PubMed


Database entries

  • Structure: 1B93 (from Escherichia coli, 50% identity, 73% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
    • induced by diamide stess (thiol depletion) (Spx) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • GP67 (mgsA::tet), available in Stülke lab
    • GP84 (mgsA::pX2(cat)), available in Stülke lab
  • Expression vector:
    • pGP1301 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Stülke lab
    • pGP1180 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • pGP1181 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Stülke lab
    • pGP2207 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP1459), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • FLAG-tag construct: GP86 (spc, based on pGP1331), available in the Stülke lab
  • Antibody:

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Your additional remarks

References

Ahmed Gaballa, Haike Antelmann, Chris J Hamilton, John D Helmann
Regulation of Bacillus subtilis bacillithiol biosynthesis operons by Spx.
Microbiology (Reading): 2013, 159(Pt 10);2025-2035
[PubMed:23894131] [WorldCat.org] [DOI] (I p)

Rachit Jain, Yajun Yan
Dehydratase mediated 1-propanol production in metabolically engineered Escherichia coli.
Microb Cell Fact: 2011, 10;97
[PubMed:22074179] [WorldCat.org] [DOI] (I e)

Jens J Landmann, Ricarda A Busse, Jan-Hendrik Latz, Kalpana D Singh, Jörg Stülke, Boris Görke
Crh, the paralogue of the phosphocarrier protein HPr, controls the methylglyoxal bypass of glycolysis in Bacillus subtilis.
Mol Microbiol: 2011, 82(3);770-87
[PubMed:21992469] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)

D Saadat, D H Harrison
The crystal structure of methylglyoxal synthase from Escherichia coli.
Structure: 1999, 7(3);309-17
[PubMed:10368300] [WorldCat.org] [DOI] (P p)