Difference between revisions of "Mfd"

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(Extended information on the protein)
(Biological materials)
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* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
  
* '''GFP fusion:'''
+
* '''GFP fusion:''' GP1510 (spc, based on [[pGP1870]]), available in the [[Stülke]] lab
 +
 
 +
* '''YFP fusion:''' GP1511 (spc, based on [[pGP1871]]), available in the [[Stülke]] lab
  
 
* '''two-hybrid system:'''  
 
* '''two-hybrid system:'''  
  
 
* '''Antibody:'''
 
* '''Antibody:'''
 +
 +
* '''Strep-tag construct:''' GP890 (spc, based on [[pGP1389]]), available in the [[Stülke]] lab
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=

Revision as of 07:33, 12 October 2011

  • Description: transcription-repair coupling factor

Gene name mfd
Synonyms
Essential no
Product transcription-repair coupling factor
Function promotes strand-specific DNA repair by displacing

RNA polymerase stalled at a nucleotide lesion and directing

the (A)BC excinuclease to the RNA damage site

Interactions involving this protein in SubtInteract: Mfd
MW, pI 133 kDa, 5.367
Gene length, protein length 3531 bp, 1177 aa
Immediate neighbours fin, spoVT
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Mfd context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

DNA repair/ recombination, transcription

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00550

Phenotypes of a mutant

In an mfd knock-out, the cell's ability to accumulate adaptive mutations in stationary phase is depressed. PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • promotes strand-specific DNA repair by displacing RNA polymerase stalled at a nucleotide lesion and directing the (A)BC excinuclease to the RNA damage site
    • is required for roadblock transcription repression by transcription factors with binding sites downstream of the promoter (as for CcpA PubMed and CodY PubMed)
  • Protein family:
  • Paralogous protein(s): RecG

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
    • 2EYQ (Mfd from E. coli) PubMed
    • 3MLQ (RNA polymerase interacting domain of Thermus thermophilus Mfd with the Thermus aquaticus RpoB beta1 domain) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1167 (del ermC), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion: GP1510 (spc, based on pGP1870), available in the Stülke lab
  • YFP fusion: GP1511 (spc, based on pGP1871), available in the Stülke lab
  • two-hybrid system:
  • Antibody:
  • Strep-tag construct: GP890 (spc, based on pGP1389), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References

Reviews


Original Articles

Additional publications: PubMed