MetS

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  • Description: methionyl-tRNA synthetase

Gene name metS
Synonyms
Essential yes PubMed
Product methionyl-tRNA synthetase
Function translation
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 76 kDa, 4.958
Gene length, protein length 1992 bp, 664 aa
Immediate neighbours abrB, yabD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MetS context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU00380

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met) (according to Swiss-Prot)
  • Protein family: tRNA-binding domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 1WE0 (from Thermus thermophilus hb8 mutant, 40% identity, 57% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon: metS
  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)