Difference between revisions of "MetS"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 1310 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 4911 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:47, 17 April 2014

  • Description: methionyl-tRNA synthetase

Gene name metS
Synonyms
Essential yes PubMed
Product methionyl-tRNA synthetase
Function translation
Gene expression levels in SubtiExpress: metS
Metabolic function and regulation of this protein in SubtiPathways:
metS
MW, pI 76 kDa, 4.958
Gene length, protein length 1992 bp, 664 aa
Immediate neighbours abrB, yabD
Sequences Protein DNA DNA_with_flanks
Genetic context
MetS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MetS expression.png




























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

  • Locus tag: BSU00380

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met) (according to Swiss-Prot)
  • Protein family: tRNA-binding domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on Ser-652 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1WE0 (from Thermus thermophilus hb8 mutant, 40% identity, 57% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon: metS
  • Sigma factor:
  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1310 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 4911 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Boumediene Soufi, Chanchal Kumar, Florian Gnad, Matthias Mann, Ivan Mijakovic, Boris Macek
Stable isotope labeling by amino acids in cell culture (SILAC) applied to quantitative proteomics of Bacillus subtilis.
J Proteome Res: 2010, 9(7);3638-46
[PubMed:20509597] [WorldCat.org] [DOI] (I p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)