melA

168

alpha-galactosidase

locus: BSU_30300

Molecular weight: 49.10 kDa

pI: 5.84

Protein length: 432 aa

Gene length: 1299 bp

function: utilization of melibiose and raffinose family oligosaccharides (raffinose, stachyose)

product: alpha-galactosidase

essential: no

ec: 3.2.1.22

synonyms: melA

List of homologs in different organisms, belongs to COG1486 (Galperin et al., 2021)

Gene

Coordinates: 3,100,881 → 3,102,179

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The protein

Catalyzed reaction/ biological activity

• Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids (according to UniProt)

Protein family

• [wiki|Glycosyl hydrolase 4 family] (according to UniProt)

[wiki|Cofactors]

• NAD⁺ [pubmed|31138628]
• Mn²⁺ [pubmed|31138628]

Structure

• [PDB|5C3M] (from Geobacillus stearothermophilus, 28% identity)
• [AF|O34645]

Kinetic information

• K_M for melibiose: 10 mM [pubmed|31138628]
• K_M for raffinose: 25 mM [pubmed|31138628]

Biological materials

Mutant

• BKE30300 (Δ[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCATATTGCTTCCCCCTT,  downstream forward: _UP4_TAAAAACTAGGGGACCGCTC
• BKK30300 (Δ[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCATATTGCTTCCCCCTT,  downstream forward: _UP4_TAAAAACTAGGGGACCGCTC

References

• 9387221,22383849,22900538,31138628