Difference between revisions of "MdtP"

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(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''  
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* '''Mutant:''' BP70 (spc) available in [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 12:28, 5 December 2012

  • Description: multidrug-efflux transporter

Gene name mdtP
Synonyms yusP
Essential no
Product multidrug-efflux transporter
Function multidrug resistance
Gene expression levels in SubtiExpress: mdtP
MW, pI 57 kDa, 9.836
Gene length, protein length 1623 bp, 541 aa
Immediate neighbours mdtR, yusQ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YusP context.gif



Categories containing this gene/protein

transporters/ other, resistance against toxins/ antibiotics, membrane proteins

This gene is a member of the following regulons

MdtR regulon

The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: EmrB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant: BP70 (spc) available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ji-Yun Kim, Takashi Inaoka, Kazutaka Hirooka, Hiroshi Matsuoka, Makiko Murata, Reiko Ohki, Yoshikazu Adachi, Yasutaro Fujita, Kozo Ochi
Identification and characterization of a novel multidrug resistance operon, mdtRP (yusOP), of Bacillus subtilis.
J Bacteriol: 2009, 191(10);3273-81
[PubMed:19286808] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)