mdh

168

malate dehydrogenase

locus: BSU_29120

Molecular weight: 33.49 kDa

pI: 4.73

Protein length: 312 aa

Gene length: 939 bp

function: TCA cycle

product: malate dehydrogenase

essential: no

ec: 1.1.1.37

synonyms: mdh, citH

List of homologs in different organisms, belongs to COG0039 (Galperin et al., 2021)

Gene

Coordinates: 2,978,734 2,979,672

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The protein

Catalyzed reaction/ biological activity

• (S)-malate + NAD⁺ --> H⁺ + NADH + oxaloacetate (according to UniProt)

Protein family

• LDH/MDH superfamily (with [protein|A0DD68FE90FD13DF03496321AB2DAAADF9F4230D|ldh], according to UniProt)

[wiki|Cofactors]

• NAD⁺

Structure

• [PDB|3TL2] (from ''B. anthracis'', 85% identity)
• [AF|P49814]

Modification

• phosphorylated on Arg-156 [Pubmed|22517742]
• phosphorylation on Ser-149 [Pubmed|17218307]

Effectors of protein activity

• Inhibited by Mg2+, Ca2+, Zn2+, Ag2+ and Hg2+ [Pubmed|14284712] [Pubmed|922015]
• Inhibited by oxaloacetate (above 1mM) and malate (above 7,7mM) [Pubmed|14284712]

[wiki|Localization]

• cytoplasm (according to UniProt)
• membrane associated [Pubmed|18763711]

Additional information

• The enzyme is a tetramer [Pubmed|14284712]
• extensive information on the structure and enzymatic properties of Mdh can be found at [http://www.proteopedia.org/wiki/index.php/Malate_dehydrogenase Proteopedia]
• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• GP719(spc) & GP1150(spc), available in [wiki|Jörg Stülke]'s lab [pubmed|20933603]
• GP790 (''[gene|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ]-[gene|CB7D0E32C044D541CD966DC1F9DA489D518A7703|icd]-[gene|5832127A2D0BD4AF2E891C93DC7C45221055FFC5|mdh]'')::''kan'', available in [wiki|Jörg Stülke]'s lab
• GP2331 (''[gene|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ]-[gene|CB7D0E32C044D541CD966DC1F9DA489D518A7703|icd]-[gene|5832127A2D0BD4AF2E891C93DC7C45221055FFC5|mdh]'')::''kan'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'',available in [wiki|Jörg Stülke]'s lab
• GP2333 (''[gene|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ]-[gene|CB7D0E32C044D541CD966DC1F9DA489D518A7703|icd]-[gene|5832127A2D0BD4AF2E891C93DC7C45221055FFC5|mdh]'')::''lox72'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [wiki|Jörg Stülke]'s lab
• BKE29120 ([gene|5832127A2D0BD4AF2E891C93DC7C45221055FFC5|mdh]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCTCTTCTCCTTTA, downstream forward: _UP4_TAATAAAAAGAGAGAAAGGC
• BKK29120 ([gene|5832127A2D0BD4AF2E891C93DC7C45221055FFC5|mdh]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCTCTTCTCCTTTA, downstream forward: _UP4_TAATAAAAAGAGAGAAAGGC

Expression vectors

• pGP385: for expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844], available in [wiki|Jörg Stülke]'s lab
• pGP1123 (N-terminal Strep-tag, for [wiki|SPINE], purification from ''B. subtilis'', in [wiki|pGP380]) (available in [wiki|Jörg Stülke]'s lab) [pubmed|20933603]
• pGP1755 (expression / purification of Mdh-S149A, with N-terminal His-tag from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
• pGP1764 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab)
• GP1438(''mdh''-''Strep'' ''(spc)'') & GP1440(''mdh''-''Strep'' ''(cat)''), purification from ''B. subtilis'', for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab

FLAG-tag construct

• GP1130 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab [pubmed|20933603]

Antibody

• **

GFP fusion

• GP1431 (spc, based on [wiki|pGP1870]), available in [wiki|Jörg Stülke]'s lab

References

• 36815589,10656796,18763711,14284712,922015,12100558,17218307,8550482,8045899,20933603,22517742,23136871,24325460,15378759,24571712,29546354,36534341