mcsB
168
protein arginine kinase, adaptor protein, forms a gated kinase chamber to mark aberrant bacterial proteins for degradation by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP], modulator of [protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR]-dependent repression
locus
BSU_00850
Molecular weight
40.97 kDa
pI
5.07
function
control of [protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR] activity
product
protein arginine kinase
essential
no
synonyms
mcsB, yacI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3869 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
102,484 103,575
Phenotypes of a mutant
more rapid spore germination as compared to wild type cells [pubmed|31221751]
The protein
Catalyzed reaction/ biological activity
phosphorylates and thereby targets non-functional [protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR] for degradation by [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]/[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] [Pubmed|20852588]
phosphorylates and thereby targets [protein|6A38DAA96F7BBF31FD8A4018A8CA7A72F3C28F79|mgsR] for degradation by [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]/[protein|297F53DAD3351E0C55108DD2C93B78FFB174438C|clpX] [Pubmed|32477307]
forms a gated kinase chamber to mark aberrant bacterial proteins for degradation by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP] [pubmed|34328418]
ATP + L-arginyl-[protein] --> ADP + H+ + Nω-phospho-L-arginyl-[protein] (according to UniProt)
Protein family
ATP:guanido phosphotransferase family (single member, according to UniProt)
[wiki|Domains]
Phosphagen kinase C-terminal (aa 24-254) (according to UniProt)
Structure
[PDB|6TV6] (octameric [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]) [pubmed|34328418]
[PDB|6FH1] (from Geobacillus stearothermophilus) [pubmed|30962626]
[PDB|6FH3] (pArg-bound state, from Geobacillus stearothermophilus) [pubmed|30962626]
[AF|P37570]
Modification
autophosphorylation on specific arginine residues [Pubmed|20852588,21622759], dephosphorylation by [protein|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE] [Pubmed|20852588,21622759]
autophosphorylation stimulates [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] activity as adaptor protein for [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] [Pubmed|21622759]
[wiki|Localization]
cytoplasm (according to UniProt)
Biological materials
Mutant
MGNA-B930 (yacI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1929 NBRP B. subtilis, Japan]
''mcsB::aphA3'' availbale from the Gerth lab
mcsBC167S::spec available from the Gerth lab
GP1457 (''mcsB''::''aphA3''), available in [wiki|Jörg Stülke]'s lab
BP69 (spc), available in [wiki|Fabian Commichau]'s lab
BKE00850 ([gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGAATAAAATGCTTTAGCG, downstream forward: _UP4_AAAAGACAGGAGGATGAATC
BKK00850 ([gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGAATAAAATGCTTTAGCG, downstream forward: _UP4_AAAAGACAGGAGGATGAATC
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
expression of native ''mcsB'' in ''B. subtilis'': pBP186 (in [wiki|pBQ200]), available in [wiki|Fabian Commichau]'s lab
Antibody
available in Gerth lab
labs
[wiki|Ulf Gerth], Greifswald, Germany
References
Reviews
Original Publications
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Time of last update: 2024-04-24 01:37:00
Author of last update: Jstuelk