Difference between revisions of "McpA"

From SubtiWiki
Jump to: navigation, search
(Extended information on the protein)
Line 83: Line 83:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:''' cell membrane (according to Swiss-Prot), membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
+
* '''Localization:''' cell membrane {{PubMed|18763711,21515776}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 10:50, 26 April 2011

  • Description: membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein

Gene name mcpA
Synonyms
Essential no
Product methyl-accepting chemotaxis protein
Function control of chemotaxis
MW, pI 72 kDa, 4.988
Gene length, protein length 1983 bp, 661 aa
Immediate neighbours tlpB, tlpA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
McpA context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

  • Locus tag: BSU31240

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): McpB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • in minimal medium, McpA is present with 15,900 +/- 3,000 molecules per cell PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Vincent J Cannistraro, George D Glekas, Christopher V Rao, George W Ordal
Cellular stoichiometry of the chemotaxis proteins in Bacillus subtilis.
J Bacteriol: 2011, 193(13);3220-7
[PubMed:21515776] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

D W Hanlon, G W Ordal
Cloning and characterization of genes encoding methyl-accepting chemotaxis proteins in Bacillus subtilis.
J Biol Chem: 1994, 269(19);14038-46
[PubMed:8188684] [WorldCat.org] (P p)

D W Hanlon, C Ying, G W Ordal
Purification and reconstitution of the methyl-accepting chemotaxis proteins from Bacillus subtilis.
Biochim Biophys Acta: 1993, 1158(3);345-51
[PubMed:8251536] [WorldCat.org] [DOI] (P p)

M S Thoelke, J M Casper, G W Ordal
Methyl group turnover on methyl-accepting chemotaxis proteins during chemotaxis by Bacillus subtilis.
J Biol Chem: 1990, 265(4);1928-32
[PubMed:2105313] [WorldCat.org] (P p)

M S Thoelke, J R Kirby, G W Ordal
Novel methyl transfer during chemotaxis in Bacillus subtilis.
Biochemistry: 1989, 28(13);5585-9
[PubMed:2505839] [WorldCat.org] [DOI] (P p)

J A Ahlgren, G W Ordal
Methyl esterification of glutamic acid residues of methyl-accepting chemotaxis proteins in Bacillus subtilis.
Biochem J: 1983, 213(3);759-63
[PubMed:6137212] [WorldCat.org] [DOI] (P p)