Difference between revisions of "ManR"

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(References)
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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of mannose utilization
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of mannose utilization
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU12000 manR]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU12000 manR]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/ManR ManR]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/ManR ManR]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjdB]]'', ''[[manP]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjdB]]'', ''[[manP]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13057&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU12000 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU12000 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU12000 Advanced_DNA]
 
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Revision as of 12:34, 13 May 2013

  • Description: transcriptional activator of the manP-manA-yjdF operon

Gene name manR
Synonyms yjdC
Essential no
Product transcriptional activator, PRD-type
Function regulation of mannose utilization
Gene expression levels in SubtiExpress: manR
Interactions involving this protein in SubtInteract: ManR
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 75 kDa, 8.04
Gene length, protein length 1944 bp, 648 aa
Immediate neighbours yjdB, manP
Sequences Protein DNA Advanced_DNA
Genetic context
ManR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ManR expression.png



















Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, ManR regulon

The ManR regulon:

The gene

Basic information

  • Locus tag: BSU12000

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation by ManP inactivates ManR (in the absence of mannose), phosphorylation by HPr stimluates ManR PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
    • ManR: transcription activation in the presence of mannose and absence of glucose PubMed
    • CcpA: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Marian Wenzel, Josef Altenbuchner
The Bacillus subtilis mannose regulator, ManR, a DNA-binding protein regulated by HPr and its cognate PTS transporter ManP.
Mol Microbiol: 2013, 88(3);562-76
[PubMed:23551403] [WorldCat.org] [DOI] (I p)

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Tianqi Sun, Josef Altenbuchner
Characterization of a mannose utilization system in Bacillus subtilis.
J Bacteriol: 2010, 192(8);2128-39
[PubMed:20139185] [WorldCat.org] [DOI] (I p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

J Stülke, M Arnaud, G Rapoport, I Martin-Verstraete
PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria.
Mol Microbiol: 1998, 28(5);865-74
[PubMed:9663674] [WorldCat.org] [DOI] (P p)