malS

168

malate dehydrogenase (decarboxylating), forms a transhydrogenation cycle with [protein|search|YtsJ ]for balancing of NADPH

locus: BSU_29880

Molecular weight: 61.98 kDa

pI: 4.89

Protein length: 566 aa

Gene length: 1701 bp

function: malate utilization

product: malate dehydrogenase (decarboxylating)

essential: no

ec: 1.1.1.38

synonyms: malS

List of homologs in different organisms, belongs to COG0281 (Galperin et al., 2021)

Gene

Coordinates: 3,056,849 → 3,058,549

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The protein

Catalyzed reaction/ biological activity

• (S)-malate + NAD+ --> CO₂ + NADH + pyruvate (according to UniProt)
• H⁺ + oxaloacetate --> CO₂ + pyruvate (according to UniProt)

Protein family

• [wiki|malic enzymes family] (according to UniProt)

Structure

• [PDB|1LLQ] (from ''Ascaris suum'', 40% identity) [Pubmed|12033925]
• [AF|O34389]

Paralogous protein(s)

• [protein|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA], [protein|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ], [protein|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]

Biological materials

Mutant

• GP1142 (Δ[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]::spc) available in [wiki|Jörg Stülke]'s lab
• BKE29880 (Δ[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCCTGCCTCTTCCTTTCT,  downstream forward: _UP4_TAATAAAAAAGAGCCGCTGC
• BKK29880 (Δ[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCCTGCCTCTTCCTTTCT,  downstream forward: _UP4_TAATAAAAAAGAGCCGCTGC

References

• 9387221,16788182,12949160,23136871,22740702,12033925,32759333