malA

168

6-phospho-alpha-glucosidase

locus: BSU_08180

Molecular weight: 50.35 kDa

pI: 4.74

Protein length: 449 aa

Gene length: 1350 bp

function: maltose utilization

product: 6-phospho-alpha-glucosidase

essential: no

ec: 3.2.1.122

synonyms: malA, glvG, glvA, mdxK

List of homologs in different organisms, belongs to COG1486 (Galperin et al., 2021)

Gene

Coordinates: 890,022 → 891,371

Advertisement

The protein

Catalyzed reaction/ biological activity

• α-maltose 6'-phosphate + H₂O --> D-glucose + D-glucose 6-phosphate (according to UniProt)

Protein family

• [wiki|Glycosyl hydrolase 4 family] (according to UniProt)

Structure

• [PDB|1U8X]
• [AF|P54716]

[wiki|Localization]

• membrane associated [Pubmed|18763711]

Biological materials

Mutant

• MGNA-C289 (glvA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2287 NBRP B. subtilis, Japan]
• BKE08180 (Δ[gene|C650160C9E5B5F8587CA9E80A57BCCE97D701037|malA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGACGACCTCCTTGAT,  downstream forward: _UP4_TAAATGAAATTCCCCCATTT
• BKK08180 (Δ[gene|C650160C9E5B5F8587CA9E80A57BCCE97D701037|malA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGACGACCTCCTTGAT,  downstream forward: _UP4_TAAATGAAATTCCCCCATTT

References

• 22900538,16707683,10627040,18763711,9765262,11489864,17676871,15341727,20618987