Difference between revisions of "Maf"

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|style="background:#ABCDEF;" align="center"|'''Function''' || cell division control
 
|style="background:#ABCDEF;" align="center"|'''Function''' || cell division control
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU28050 maf]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28050 maf]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Maf Maf]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Maf Maf]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[radC]]'', ''[[spoIIB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[radC]]'', ''[[spoIIB]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14765&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28050 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:maf_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:maf_context.gif]]

Revision as of 13:29, 13 May 2013

  • Description: cell division inhibitor in competent cells, blocks septation during the escape from competence, required for the stability of ComGA

Gene name maf
Synonyms
Essential no
Product cell division inhibitor
Function cell division control
Gene expression levels in SubtiExpress: maf
Interactions involving this protein in SubtInteract: Maf
MW, pI 21 kDa, 5.711
Gene length, protein length 567 bp, 189 aa
Immediate neighbours radC, spoIIB
Sequences Protein DNA Advanced_DNA
Genetic context
Maf context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Maf expression.png
























Categories containing this gene/protein

cell division

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

  • Locus tag: BSU28050

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: maf family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): binds phosphorylated compounds (UTP, IMP, IDP, GDP and others) in in vitro experiments
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • expressed when cells enter the compentent state (ComK) PubMed
    • expression is reduced in a SigV mutant PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

PubMed

Original publications

Additional publications: PubMed

Kenneth Briley, Peter Prepiak, Miguel J Dias, Jeanette Hahn, David Dubnau
Maf acts downstream of ComGA to arrest cell division in competent cells of B. subtilis.
Mol Microbiol: 2011, 81(1);23-39
[PubMed:21564336] [WorldCat.org] [DOI] (I p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)

G Minasov, M Teplova, G C Stewart, E V Koonin, W F Anderson, M Egli
Functional implications from crystal structures of the conserved Bacillus subtilis protein Maf with and without dUTP.
Proc Natl Acad Sci U S A: 2000, 97(12);6328-33
[PubMed:10841541] [WorldCat.org] [DOI] (P p)

Y X Butler, Y Abhayawardhane, G C Stewart
Amplification of the Bacillus subtilis maf gene results in arrested septum formation.
J Bacteriol: 1993, 175(10);3139-45
[PubMed:8387996] [WorldCat.org] [DOI] (P p)