MaeN

From SubtiWiki
Revision as of 16:35, 13 July 2013 by Jstuelk (talk | contribs) (Original publications)
Jump to: navigation, search
  • Description: Na+/malate symporter

Gene name maeN
Synonyms yufR
Essential no
Product Na+/malate symporter
Function malate uptake
Gene expression levels in SubtiExpress: maeN
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 47 kDa, 8.411
Gene length, protein length 1344 bp, 448 aa
Immediate neighbours nupQ, yufS
Sequences Protein DNA DNA_with_flanks
Genetic context
MaeN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MaeN expression.png




























Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

MalR regulon

The gene

Basic information

  • Locus tag: BSU31580

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: sodium:citrate (SCF) symporter family (according to Swiss-Prot) 2-hydroxycarboxylate transporter family
  • Paralogous protein(s): CimH

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: induced by malate (MalR)
  • Regulatory mechanism: MalR: transcription activation
  • Additional information:
    • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

  • Mutant:
  • Expression vector:
    • expression of native maeN in B. subtilis: pGP1951 (in pBQ200), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Iwona Sobczak, Juke S Lolkema
The 2-hydroxycarboxylate transporter family: physiology, structure, and mechanism.
Microbiol Mol Biol Rev: 2005, 69(4);665-95
[PubMed:16339740] [WorldCat.org] [DOI] (P p)

Original publications