Difference between revisions of "LytE"

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* a ''[[cwlO]] [[lytE]]'' mutant is not viable {{PubMed|17581128,22139507}}
 
* a ''[[cwlO]] [[lytE]]'' mutant is not viable {{PubMed|17581128,22139507}}
 
* growth defect at high temperature {{PubMed|21541672}}
 
* growth defect at high temperature {{PubMed|21541672}}
* inactivation of ''[[lytE]]'' strongly restores beta-lactam resistance in a ''[[sigM]]'' mutant {{PubMed|22211522}}
+
* inactivation of ''[[lytE]]'' strongly restores beta-lactam resistance in a ''[[sigM]]'' mutant by delaying cell lysis {{PubMed|22211522}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
** [[MreBH]]-[[LytE]] {{PubMed|16950129}}
+
** [[MreBH]]-[[LytE]] along the cylindrical part of the cell wall {{PubMed|16950129}}
 +
** [[FtsZ]]-[[LytE]] at the division septum {{PubMed|16950129}}
 +
** [[PbpB]]-[[LytE]] at the division septum {{PubMed|16950129}}
  
 
* '''[[Localization]]:'''  
 
* '''[[Localization]]:'''  

Revision as of 19:50, 21 July 2012

  • Description: cell wall hydrolase (major autolysin), D,L-endopeptidase-type autolysin

Gene name lytE
Synonyms papQ, cwlF
Essential no
Product cell wall hydrolase (major autolysin),endopeptidase-type autolysin
Function major autolysin, cell separation, cell proliferation
MW, pI 37 kDa, 10.713
Gene length, protein length 1029 bp, 343 aa
Immediate neighbours phoA, citR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LytE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LytE expression.png















Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

SigH regulon, SigI regulon, Spo0A regulon, WalR regulon

The gene

Basic information

  • Locus tag: BSU09420

Phenotypes of a mutant

  • a cwlO lytE mutant is not viable PubMed
  • growth defect at high temperature PubMed
  • inactivation of lytE strongly restores beta-lactam resistance in a sigM mutant by delaying cell lysis PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: nlpC/p60 family (according to Swiss-Prot)
  • Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

  • Kinetic information:
  • Domains:
    • C-terminal D,L-endopeptidase domain PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • secreted (according to Swiss-Prot)
    • binds the cell wall PubMed
    • localizes to cell septa, poles and lateral sidewall of the cell (via the N-terminal domain) PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
    • induced at high temperature (SigI) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed