Difference between revisions of "LytE"

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(References)
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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
 
{{SubtiWiki regulon|[[SigH regulon]]}},
 
{{SubtiWiki regulon|[[SigH regulon]]}},
 +
{{SubtiWiki regulon|[[SigI regulon]]}},
 
{{SubtiWiki regulon|[[Spo0A regulon]]}},
 
{{SubtiWiki regulon|[[Spo0A regulon]]}},
 
{{SubtiWiki regulon|[[WalR regulon]]}}
 
{{SubtiWiki regulon|[[WalR regulon]]}}
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
a ''[[cwlO]] [[lytE]]'' mutant is not viable {{PubMed|17581128}}
+
** a ''[[cwlO]] [[lytE]]'' mutant is not viable {{PubMed|17581128}}
 +
** growth defect at high temperature {{PubMed|21541672}}
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
 
 
  
  
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* '''Operon:''' ''lytE'' {{PubMed|9573210}}
 
* '''Operon:''' ''lytE'' {{PubMed|9573210}}
  
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9573210}}, [[SigH]] {{PubMed|9573210}}
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9573210}}, [[SigH]] {{PubMed|9573210}}, [[SigI]] {{PubMed|21541672}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
 
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
 
+
** induced at high temperature ([[SigI]]) {{PubMed|21541672}}
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 
** [[WalR]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/17581128 PubMed]
 
** [[WalR]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/17581128 PubMed]
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=References=
 
=References=
 
'''Additional publications:''' {{PubMed|21261835}}
 
'''Additional publications:''' {{PubMed|21261835}}
<pubmed>16950129,17581128,14594841,9457885,9573210,10322020, 20059685,14651647,</pubmed>
+
<pubmed>16950129,17581128,14594841,9457885,9573210,10322020, 20059685,14651647, 21541672 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 13:51, 5 May 2011

  • Description: cell wall hydrolase (major autolysin),endopeptidase-type autolysin

Gene name lytE
Synonyms papQ, cwlF
Essential no
Product cell wall hydrolase (major autolysin),endopeptidase-type autolysin
Function major autolysin, cell separation, cell lysis
MW, pI 37 kDa, 10.713
Gene length, protein length 1029 bp, 343 aa
Immediate neighbours phoA, citR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LytE context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

SigH regulon, SigI regulon, Spo0A regulon, WalR regulon

The gene

Basic information

  • Locus tag: BSU09420

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: nlpC/p60 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: secreted (according to Swiss-Prot), binds the cell wall PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
    • induced at high temperature (SigI) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Chi-Ling Tseng, Jung-Tze Chen, Ju-Hui Lin, Wan-Zhen Huang, Gwo-Chyuan Shaw
Genetic evidence for involvement of the alternative sigma factor SigI in controlling expression of the cell wall hydrolase gene lytE and contribution of LytE to heat survival of Bacillus subtilis.
Arch Microbiol: 2011, 193(9);677-85
[PubMed:21541672] [WorldCat.org] [DOI] (I p)

Paola Bisicchia, David Noone, Efthimia Lioliou, Alistair Howell, Sarah Quigley, Thomas Jensen, Hanne Jarmer, Kevin M Devine
The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis.
Mol Microbiol: 2007, 65(1);180-200
[PubMed:17581128] [WorldCat.org] [DOI] (P p)

Rut Carballido-López, Alex Formstone, Ying Li, S Dusko Ehrlich, Philippe Noirot, Jeff Errington
Actin homolog MreBH governs cell morphogenesis by localization of the cell wall hydrolase LytE.
Dev Cell: 2006, 11(3);399-409
[PubMed:16950129] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Hiroki Yamamoto, Shin-ichirou Kurosawa, Junichi Sekiguchi
Localization of the vegetative cell wall hydrolases LytC, LytE, and LytF on the Bacillus subtilis cell surface and stability of these enzymes to cell wall-bound or extracellular proteases.
J Bacteriol: 2003, 185(22);6666-77
[PubMed:14594841] [WorldCat.org] [DOI] (P p)

R Ohnishi, S Ishikawa, J Sekiguchi
Peptidoglycan hydrolase LytF plays a role in cell separation with CwlF during vegetative growth of Bacillus subtilis.
J Bacteriol: 1999, 181(10);3178-84
[PubMed:10322020] [WorldCat.org] [DOI] (P p)

S Ishikawa, Y Hara, R Ohnishi, J Sekiguchi
Regulation of a new cell wall hydrolase gene, cwlF, which affects cell separation in Bacillus subtilis.
J Bacteriol: 1998, 180(9);2549-55
[PubMed:9573210] [WorldCat.org] [DOI] (P p)

P Margot, M Wahlen, A Gholamhoseinian, P Piggot, D Karamata
The lytE gene of Bacillus subtilis 168 encodes a cell wall hydrolase.
J Bacteriol: 1998, 180(3);749-52
[PubMed:9457885] [WorldCat.org] [DOI] (P p)