Difference between revisions of "LysS"

From SubtiWiki
Jump to: navigation, search
Line 82: Line 82:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37477 P37477]
+
* '''UniProt:''' [http://www.uniprot.org/uniprot/P37477 P37477]
  
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00820]
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00820]

Revision as of 09:44, 20 July 2009

  • Description: lysyl-tRNA synthetase

Gene name lysS
Synonyms
Essential yes PubMed
Product lysyl-tRNA synthetase
Function translation
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging, Folate
MW, pI 57 kDa, 5.034
Gene length, protein length 1497 bp, 499 aa
Immediate neighbours yacF, rrnJ-16S
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LysS context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU00820

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys) (according to Swiss-Prot)
  • Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

lysS PubMed

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Antoine de Saizieu, Pierre Vankan, Cassandra Vockler, Adolphus P G M van Loon
The trp RNA-binding attenuation protein (TRAP) regulates the steady-state levels of transcripts of the Bacillus subtilis folate operon.
Microbiology (Reading): 1997, 143 ( Pt 3);979-989
[PubMed:9084182] [WorldCat.org] [DOI] (P p)