LysA

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  • Description: diaminopimelate decarboxylase

Gene name lysA
Synonyms
Essential no
Product diaminopimelate decarboxylase
Function biosynthesis of lysine
Gene expression levels in SubtiExpress: lysA
Metabolic function and regulation of this protein in SubtiPathways:
lysA
MW, pI 48 kDa, 5.007
Gene length, protein length 1317 bp, 439 aa
Immediate neighbours ypuA, spoVAF
Sequences Protein DNA DNA_with_flanks
Genetic context
LysA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LysA expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

  • Locus tag: BSU23380

Phenotypes of a mutant

  • auxotrophic for lysine PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Meso-2,6-diaminoheptanedioate = L-lysine + CO2 (according to Swiss-Prot)
  • Protein family: Orn/Lys/Arg decarboxylase class-II family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 1HKW (from Mycobacterium tuberculosis, 42% identity, 61% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 627 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 2286 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Alain Brans, Patrice Filée, Andy Chevigné, Aurore Claessens, Bernard Joris
New integrative method to generate Bacillus subtilis recombinant strains free of selection markers.
Appl Environ Microbiol: 2004, 70(12);7241-50
[PubMed:15574923] [WorldCat.org] [DOI] (P p)

I Bagyan, J Hobot, S Cutting
A compartmentalized regulator of developmental gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(15);4500-7
[PubMed:8755877] [WorldCat.org] [DOI] (P p)

V Azevedo, A Sorokin, S D Ehrlich, P Serror
The transcriptional organization of the Bacillus subtilis 168 chromosome region between the spoVAF and serA genetic loci.
Mol Microbiol: 1993, 10(2);397-405
[PubMed:7934830] [WorldCat.org] [DOI] (P p)

B Moldover, P J Piggot, M D Yudkin
Identification of the promoter and the transcriptional start site of the spoVA operon of Bacillus subtilis and Bacillus licheniformis.
J Gen Microbiol: 1991, 137(3);527-31
[PubMed:1903432] [WorldCat.org] [DOI] (P p)