lysA

168

diaminopimelate decarboxylase

locus: BSU_23380

Molecular weight: 48.41 kDa

pI: 5.01

Protein length: 439 aa

Gene length: 1317 bp

function: biosynthesis of lysine

product: diaminopimelate decarboxylase

essential: no

ec: 4.1.1.20

synonyms: lysA

List of homologs in different organisms, belongs to COG0019 (Galperin et al., 2021)

Gene

Coordinates: 2,436,947 → 2,438,266

Phenotypes of a mutant

• auxotrophic for lysine [Pubmed|15574923]
• reduced conjugation of ICEBs1 [Pubmed|25069588]

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The protein

Catalyzed reaction/ biological activity

• H⁺ + meso-2,6-diaminopimelate --> CO₂ + L-lysine (according to UniProt)

Protein family

• Orn/Lys/Arg decarboxylase class-II family (single member, according to UniProt)

[wiki|Cofactors]

• PLP (according to UniProt)

Structure

• [PDB|1HKW] (from ''Mycobacterium tuberculosis'', 42% identity, 61% similarity) [Pubmed|12637582]
• [AF|P23630]

Biological materials

Mutant

• 1A615 ( ''lysA''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A615&Search=1A615 BGSC]
• BKE23380 (Δ[gene|02F1C926D200DE85C93A0ACEF23AD25FBD12B0BD|lysA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23380 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGTCATTCCCTCTTTCT,  downstream forward: _UP4_TAAAAGAAAGCGCCGATTTT
• BKK23380 (Δ[gene|02F1C926D200DE85C93A0ACEF23AD25FBD12B0BD|lysA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23380 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGTCATTCCCTCTTTCT,  downstream forward: _UP4_TAAAAGAAAGCGCCGATTTT

References

• 7934830,15574923,15699190,1903432,8755877,25069588