Difference between revisions of "LuxS"

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** number of protein molecules per cell (minimal medium with glucose and ammonium): 2174 {{PubMed|24696501}}
 
** number of protein molecules per cell (minimal medium with glucose and ammonium): 2174 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 3894 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 3894 {{PubMed|24696501}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 2289 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1319 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2360 {{PubMed|21395229}}
  
 
=Biological materials =
 
=Biological materials =
 
 
* '''Mutant:'''
 
* '''Mutant:'''
 
** 1A953 ( ''luxS''::''cat''), {{PubMed|17056751}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A953&Search=1A953 BGSC]
 
** 1A953 ( ''luxS''::''cat''), {{PubMed|17056751}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A953&Search=1A953 BGSC]

Revision as of 14:09, 17 April 2014

  • Description: S-ribosylhomocysteine lyase, autoinducer-2 production protein, required for swarming motility and biofilm formation

Gene name luxS
Synonyms ytjB
Essential no
Product S-ribosylhomocysteine lyase
Function methionine salvage
Gene expression levels in SubtiExpress: luxS
Metabolic function and regulation of this protein in SubtiPathways:
luxS
MW, pI 17 kDa, 5.168
Gene length, protein length 471 bp, 157 aa
Immediate neighbours ytkA, ytjA
Sequences Protein DNA DNA_with_flanks
Genetic context
LuxS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
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Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, motility and chemotaxis, quorum sensing, biofilm formation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU30670

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione (according to Swiss-Prot)
  • Protein family: luxS family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: subject to feedback inhibition PubMed

Database entries

  • Structure: 1JQW (complex with homocysteine), 1J98
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information: subject to feedback inhibition PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 2174 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 3894 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 2289 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1319 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2360 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Esteban Lombardía, Adrián J Rovetto, Ana L Arabolaza, Roberto R Grau
A LuxS-dependent cell-to-cell language regulates social behavior and development in Bacillus subtilis.
J Bacteriol: 2006, 188(12);4442-52
[PubMed:16740951] [WorldCat.org] [DOI] (P p)

M T Hilgers, M L Ludwig
Crystal structure of the quorum-sensing protein LuxS reveals a catalytic metal site.
Proc Natl Acad Sci U S A: 2001, 98(20);11169-74
[PubMed:11553770] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)