lutC

168

lactate catabolic enzyme

locus: BSU_34030

Molecular weight: 26.13 kDa

pI: 5.16

Protein length: 240 aa

Gene length: 723 bp

function: utilization of lactate

product: unknown

essential: no

ec: null

synonyms: lutC, yvbY

List of homologs in different organisms, belongs to COG1556 (Galperin et al., 2021)

Gene

Coordinates: 3,492,797  3,493,519

Phenotypes of a mutant

• no growth with lactate as the single carbon source [Pubmed|19201793]

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The protein

Catalyzed reaction/ biological activity

• oxidation of lactate to pyruvate [Pubmed|19201793]

Protein family

• LutC/YkgG family (single member, according to UniProt)

Structure

• [AF|O32259]

Modification

• phosphorylated on Arg-97 [Pubmed|22517742]

Expression and Regulation

Other regulations

• [protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|fsrA]: translation repression, [Pubmed|22427629]
• [protein|147AFEBEA546FDBEEB08E9A8D9C7BCDC9B83CC90|fbpB]: translation inhibition, acts as RNA chaperone, increases interaction between [protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|fsrA] and [protein|FEC83B66CEA311EA1650D61A215F82E9DF4E9F03|lutA]-[protein|57B9E37F6232189CD47C1B41FDCF43FCB8016AEB|lutB]-[protein|5EE69198882DF4E9AB8D9D7267F071EF88C2F16D|lutC] RNAs

Biological materials

Mutant

• MGNA-A470 (yvbY::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/470 NBRP B. subtilis, Japan]
• BKE34030 ([gene|5EE69198882DF4E9AB8D9D7267F071EF88C2F16D|lutC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATTCTGAATGGTTCCCTTCG,  downstream forward: _UP4_TGAACTCAGGAAGCCCGGCA
• BKK34030 ([gene|5EE69198882DF4E9AB8D9D7267F071EF88C2F16D|lutC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATTCTGAATGGTTCCCTTCG,  downstream forward: _UP4_TGAACTCAGGAAGCCCGGCA

labs

• [wiki|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

References

• 22427629,19201793,19201793,22517742,36815589