Difference between revisions of "List of plasmids"

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(Expression/ purification in E. coli)
(Fusion to reporter genes)
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*[[pAC7]]: translational ''lacZ''-fusions (kanamycin resistence)
 
*[[pAC7]]: translational ''lacZ''-fusions (kanamycin resistence)
 
* pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression {{PubMed|20150235}}
 
* pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression {{PubMed|20150235}}
 +
* a suite of vectors for ectopic insertion of GFP, CFP and IYFP transcriptional fusions in single copy at the ''amyE'' and ''bglS'' loci {{PubMed|20600285}}
  
 
==Labelling of a protein with a triple FLAG tag==
 
==Labelling of a protein with a triple FLAG tag==

Revision as of 05:56, 7 July 2010

Here you can find a list of Bacillus subtilis plasmids:

Fusion to reporter genes

  • pAC5: translational lacZ-fusions (chloramphenicol resistence)
  • pAC6: transcriptional lacZ-fusions (cat resistence)
  • pAC7: translational lacZ-fusions (kanamycin resistence)
  • pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression PubMed
  • a suite of vectors for ectopic insertion of GFP, CFP and IYFP transcriptional fusions in single copy at the amyE and bglS loci PubMed

Labelling of a protein with a triple FLAG tag

  • pGP1331: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
  • pGP1087: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter

Expression/ purification in B. subtilis

  • pBQ200: overexpression of proteins in B. subtilis under control of a strong degQ36 promoter
  • pGP380: expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
  • pGP382: expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein

Expression/ purification in E. coli

  • pWH844: expression of proteins in E. coli, allows fusion to a N-terminal His-tag
  • pGP570: expression of proteins in E. coli, allows fusion to a N-terminal His-tag that can be cleaved off
  • pGP172: expression of proteins in E. coli allows fusion to a Strep-tag at the N-terminus of the protein
  • pGP574: expression of proteins in E. coli allows fusion to a Strep-tag at the C-terminus of the protein