LipC

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  • Description: spore coat protein, phospholipase implicated in spore germination

Gene name lipC
Synonyms ycsK
Essential no
Product spore coat phospholipase B
Function spore germination
Gene expression levels in SubtiExpress: lipC
Function and regulation of this protein in SubtiPathways:
lipC
MW, pI 23 kDa, 5.839
Gene length, protein length 639 bp, 213 aa
Immediate neighbours kipR, yczI
Sequences Protein DNA DNA_with_flanks
Genetic context
YcsK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LipC expression.png















Categories containing this gene/protein

utilization of lipids, sporulation proteins, germination

This gene is a member of the following regulons

KipR regulon, SigK regulon, TnrA regulon, GerE regulon


The gene

Basic information

  • Locus tag: BSU04110

Phenotypes of a mutant

  • defective in L-alanine-stimulated germination PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: cleaves the fatty acids at the sn-1 and sn-2 positions of phospholipids PubMed
  • Protein family: 'GDSL' lipolytic enzyme family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • lipC: expressed four hours after onset of sporulation (GerE) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Peter T McKenney, Adam Driks, Patrick Eichenberger
The Bacillus subtilis endospore: assembly and functions of the multilayered coat.
Nat Rev Microbiol: 2013, 11(1);33-44
[PubMed:23202530] [WorldCat.org] [DOI] (I p)

Original publications

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Atsushi Masayama, Shiro Kato, Takuya Terashima, Anne Mølgaard, Hisashi Hemmi, Tohru Yoshimura, Ryuichi Moriyama
Bacillus subtilis spore coat protein LipC is a phospholipase B.
Biosci Biotechnol Biochem: 2010, 74(1);24-30
[PubMed:20057119] [WorldCat.org] [DOI] (I p)

Atsushi Masayama, Ritsuko Kuwana, Hiromu Takamatsu, Hisashi Hemmi, Tohru Yoshimura, Kazuhito Watabe, Ryuichi Moriyama
A novel lipolytic enzyme, YcsK (LipC), located in the spore coat of Bacillus subtilis, is involved in spore germination.
J Bacteriol: 2007, 189(6);2369-75
[PubMed:17220230] [WorldCat.org] [DOI] (P p)

L Wang, R Grau, M Perego, J A Hoch
A novel histidine kinase inhibitor regulating development in Bacillus subtilis.
Genes Dev: 1997, 11(19);2569-79
[PubMed:9334321] [WorldCat.org] [DOI] (P p)