LipC

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  • Description: phospholipase implicated in spore germination

Gene name lipC
Synonyms ycsK
Essential no
Product spore coat phospholipase B
Function spore germination
Function and regulation of this protein in SubtiPathways:
Phosphorelay
MW, pI 23 kDa, 5.839
Gene length, protein length 639 bp, 213 aa
Immediate neighbours kipR, yczI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YcsK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LipC expression.png




























Categories containing this gene/protein

utilization of lipids, sporulation proteins, germination

This gene is a member of the following regulons

KipR regulon, SigK regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU04110

Phenotypes of a mutant

defective in L-alanine-stimulated germination PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: cleaves the fatty acids at the sn-1 and sn-2 positions of phospholipids PubMed
  • Protein family: 'GDSL' lipolytic enzyme family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • lipC: expressed four hours after onset of sporulation (GerE) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Atsushi Masayama, Shiro Kato, Takuya Terashima, Anne Mølgaard, Hisashi Hemmi, Tohru Yoshimura, Ryuichi Moriyama
Bacillus subtilis spore coat protein LipC is a phospholipase B.
Biosci Biotechnol Biochem: 2010, 74(1);24-30
[PubMed:20057119] [WorldCat.org] [DOI] (I p)

Atsushi Masayama, Ritsuko Kuwana, Hiromu Takamatsu, Hisashi Hemmi, Tohru Yoshimura, Kazuhito Watabe, Ryuichi Moriyama
A novel lipolytic enzyme, YcsK (LipC), located in the spore coat of Bacillus subtilis, is involved in spore germination.
J Bacteriol: 2007, 189(6);2369-75
[PubMed:17220230] [WorldCat.org] [DOI] (P p)

L Wang, R Grau, M Perego, J A Hoch
A novel histidine kinase inhibitor regulating development in Bacillus subtilis.
Genes Dev: 1997, 11(19);2569-79
[PubMed:9334321] [WorldCat.org] [DOI] (P p)