lipC

168

spore coat protein, phospholipase implicated in spore germination

locus: BSU_04110

Molecular weight: 23.46 kDa

pI: 5.84

Protein length: 213 aa

Gene length: 642 bp

function: spore germination

product: spore coat phospholipase B

essential: no

synonyms: lipC, ycsK

List of homologs in different organisms, belongs to COG2755 (Galperin et al., 2021)

Gene

Coordinates: 462,431  463,072

Phenotypes of a mutant

• defective in L-alanine-stimulated germination [Pubmed|20057119]

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The protein

Catalyzed reaction/ biological activity

• cleaves the fatty acids at the sn-1 and sn-2 positions of phospholipids [Pubmed|20057119]

Protein family

• [wiki|'GDSL' lipolytic enzyme family] (according to UniProt)

Structure

• [PDB|4PPY] (from Bacteroides fragilis, corresponds to aa 41 ... 210, 25.4% identity)
• [AF|P42969]

[wiki|Localization]

• spore coat (basement), localization depends on [protein|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA] [Pubmed|22171814,17220230,20057119]

Biological materials

Mutant

• MGNA-C024 (ycsK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2022 NBRP B. subtilis, Japan]
• BKE04110 ([gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTTCCTCCACTCT,  downstream forward: _UP4_TAGAAAACGCGCCTCATGAC
• BKK04110 ([gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTTCCTCCACTCT,  downstream forward: _UP4_TAGAAAACGCGCCTCATGAC

References

Reviews

• 23202530,27227299,35638784

Original Publications

• 17220230,9334321,20057119,22171814,25755103