lipC
168
spore coat protein, phospholipase implicated in spore germination
locus
BSU_04110
Molecular weight
23.46 kDa
pI
5.84
function
spore germination
product
spore coat phospholipase B
essential
no
synonyms
lipC, ycsK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2755 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
462,431 463,072
Phenotypes of a mutant
defective in L-alanine-stimulated germination [Pubmed|20057119]
The protein
Catalyzed reaction/ biological activity
cleaves the fatty acids at the sn-1 and sn-2 positions of phospholipids [Pubmed|20057119]
Protein family
[wiki|'GDSL' lipolytic enzyme family] (according to UniProt)
Structure
[PDB|4PPY] (from Bacteroides fragilis, corresponds to aa 41 ... 210, 25.4% identity)
[AF|P42969]
[wiki|Localization]
spore coat (basement), localization depends on [protein|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA] [Pubmed|22171814,17220230,20057119]
Biological materials
Mutant
MGNA-C024 (ycsK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2022 NBRP B. subtilis, Japan]
BKE04110 ([gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTTCCTCCACTCT, downstream forward: _UP4_TAGAAAACGCGCCTCATGAC
BKK04110 ([gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTTCCTCCACTCT, downstream forward: _UP4_TAGAAAACGCGCCTCATGAC
References
Reviews
Original Publications
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Time of last update: 2024-04-24 12:05:07
Author of last update: Jstuelk