LipA

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  • Description: trigger enzyme: lipoic acid synthase and regulator of comE operon expression

Gene name lipA
Synonyms yutB
Essential Yes PubMed
Product trigger enzyme: lipoic acid synthase
Function lipid metabolism, required for the synthesis of branched-chain amino acids
Gene expression levels in SubtiExpress: lipA
Metabolic function and regulation of this protein in SubtiPathways:
Protein secretion
MW, pI 33 kDa, 8.251
Gene length, protein length 894 bp, 298 aa
Immediate neighbours yutC, lytH
Sequences Protein DNA Advanced_DNA
Genetic context
LipA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LipA expression.png
























Categories containing this gene/protein

biosynthesis of cofactors, trigger enzyme

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU32330

Phenotypes of a mutant

reduced transformation efficiency PubMed, strong inhibition of growth on minimal media PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol + H2O = diacylglycerol + a carboxylate (according to Swiss-Prot) required for comEA transcription PubMed
  • Protein family: Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Natalia Martin, Esteban Lombardía, Silvia G Altabe, Diego de Mendoza, María C Mansilla
A lipA (yutB) mutant, encoding lipoic acid synthase, provides insight into the interplay between branched-chain and unsaturated fatty acid biosynthesis in Bacillus subtilis.
J Bacteriol: 2009, 191(24);7447-55
[PubMed:19820084] [WorldCat.org] [DOI] (I p)

Mitsuo Ogura, Teruo Tanaka
The Bacillus subtilis late competence operon comE is transcriptionally regulated by yutB and under post-transcription initiation control by comN (yrzD).
J Bacteriol: 2009, 191(3);949-58
[PubMed:19028902] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)