Difference between revisions of "LipA"

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* '''Description:''' [[trigger enzymes|trigger enzyme]]: lipoic acid synthase and regulator of ''[[comEA|comE]]'' operon expression <br/><br/>
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* '''Description:''' [[trigger enzymes|trigger enzyme]]: lipoic acid synthase and regulator of ''[[comEA|comE]]'' operon expression &lt;br/>&lt;br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yutC]]'', ''[[lytH]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yutC]]'', ''[[lytH]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15223&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;#91;EMBLCDS:CAB15223&amp;#93;+-newId sequences] &lt;br/> (Barbe ''et al.'', 2009)'''
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:lipA_context.gif]]
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|colspan="2" | '''Genetic context''' &lt;br/> [[Image:lipA_context.gif]]
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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  &lt;div align="right"> &lt;small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&lt;/small>&lt;/div>
 
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__TOC__
 
__TOC__
  
<br/><br/>
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&lt;br/>&lt;br/>
  
 
=The gene=
 
=The gene=
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol + H<sub>2</sub>O = diacylglycerol + a carboxylate (according to Swiss-Prot) required for ''[[comEA]]'' transcription  [http://www.ncbi.nlm.nih.gov/sites/entrez/19028902 PubMed]
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* '''Catalyzed reaction/ biological activity:''' Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol + H&lt;sub>2&lt;/sub>O = diacylglycerol + a carboxylate (according to Swiss-Prot) required for ''[[comEA]]'' transcription  [http://www.ncbi.nlm.nih.gov/sites/entrez/19028902 PubMed]
  
 
* '''Protein family:''' Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot)
 
* '''Protein family:''' Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot)
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=References=
 
=References=
  
<pubmed>18957862 19028902, 19820084 </pubmed>
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&lt;pubmed>18957862 19028902, 19820084 &lt;/pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:24, 24 November 2010



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  • Description: trigger enzyme: lipoic acid synthase and regulator of comE operon expression <br/><br/>
Gene name lipA
Synonyms yutB
Essential Yes PubMed
Product trigger enzyme: lipoic acid synthase
Function lipid metabolism, required for the synthesis of branched-chain amino acids
MW, pI 33 kDa, 8.251
Gene length, protein length 894 bp, 298 aa
Immediate neighbours yutC, lytH
Get the DNA and protein sequences <br/> (Barbe et al., 2009)
Genetic context <br/> LipA context.gif
<div align="right"> <small>This image was kindly provided by SubtiList</small></div>

<br/><br/>

The gene

Basic information

  • Locus tag: BSU32330

Phenotypes of a mutant

reduced transformation efficiency PubMed, strong inhibition of growth on minimal media PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol + H<sub>2</sub>O = diacylglycerol + a carboxylate (according to Swiss-Prot) required for comEA transcription PubMed
  • Protein family: Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: extracellular (signal peptide) PubMed

Database entries

  • Structure:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

<pubmed>18957862 19028902, 19820084 </pubmed>