Difference between revisions of "LigA"

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=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:'''
+
* '''Locus tag:''' BSU06620
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O31498 O31498]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O31498 O31498]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU06620 BSU06620]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU06620]
  
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/6.5.1.2 6.5.1.2]
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/6.5.1.2 6.5.1.2]

Revision as of 13:55, 3 June 2009

  • Description: DNA ligase (NAD-dependent)

Gene name ligA
Synonyms lig, yerG
Essential yes PubMed
Product DNA ligase (NAD-dependent)
Function replication
MW, pI 74 kDa, 4.708
Gene length, protein length 2004 bp, 668 aa
Immediate neighbours pcrA, yerH
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LigA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU06620

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m) (according to Swiss-Prot)
  • Protein family: BRCT domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1B04 (adenylation domain, Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed