Difference between revisions of "LiaS"
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' ATP + protein L-histidine = ADP + protein N-phospho-L-histidine (according to Swiss-Prot) |
* '''Protein family:''' | * '''Protein family:''' | ||
Revision as of 13:59, 24 May 2009
- Description: two-component sensor kinase, response to bacitracin
| Gene name | liaS |
| Synonyms | yvqE |
| Essential | no |
| Product | two-component sensor kinase (NarL family) |
| Function | control of LiaR activity in response to bacitracin |
| MW, pI | 40 kDa, 7.333 |
| Gene length, protein length | 1080 bp, 360 aa |
| Immediate neighbours | liaR, liaF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- Swiss prot entry: O32198
- KEGG entry: BSU33090
- E.C. number:
Additional information
Expression and regulation
- Sigma factor: SigA
liaS: constitutive
- Regulatory mechanism: LiaR: transcription activation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
John Helmann, Cornell University, USA Homepage
Thorsten Mascher, Karlsruhe Institute of Technology, Germany link
Your additional remarks
References
- Jordan, S., A. Junker, J. D. Helmann, and T. Mascher. 2006. Regulation of LiaRS-dependent gene expression in Bacillus subtilis: identification of inhibitor proteins, regulator binding sites, and target genes of a conserved cell envelope stress-sensing two-component system. J. Bacteriol. 188: 5153-5166. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
