Difference between revisions of "LiaF"

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|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[LiaR]] activity
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[LiaR]] activity
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU33100 liaF]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33100 liaF]
 
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|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26 kDa, 9.211   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26 kDa, 9.211   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[liaS]]'', ''[[liaG]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[liaS]]'', ''[[liaG]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15300&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33100 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33100 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33100 Advanced_DNA]
 
|-
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yvqF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yvqF_context.gif]]

Revision as of 13:49, 13 May 2013

  • Description: inhibitor of LiaS kinase activity

Gene name liaF
Synonyms yvqF
Essential no
Product negative effector of LiaS
Function control of LiaR activity
Gene expression levels in SubtiExpress: liaF
MW, pI 26 kDa, 9.211
Gene length, protein length 723 bp, 241 aa
Immediate neighbours liaS, liaG
Sequences Protein DNA Advanced_DNA
Genetic context
YvqF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LiaF expression.png















Categories containing this gene/protein

transcription factors and their control, resistance against oxidative and electrophile stress, resistance against toxins/ antibiotics, membrane proteins

This gene is a member of the following regulons

LiaR regulon

The gene

Basic information

  • Locus tag: BSU33100

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • inhibitor of LiaS kinase activity, maintains LiaS in the phosphatase state in the absence of the stress signal PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Karen Schrecke, Sina Jordan, Thorsten Mascher
Stoichiometry and perturbation studies of the LiaFSR system of Bacillus subtilis.
Mol Microbiol: 2013, 87(4);769-88
[PubMed:23279150] [WorldCat.org] [DOI] (I p)

Diana Wolf, Falk Kalamorz, Tina Wecke, Anna Juszczak, Ulrike Mäder, Georg Homuth, Sina Jordan, Janine Kirstein, Michael Hoppert, Birgit Voigt, Michael Hecker, Thorsten Mascher
In-depth profiling of the LiaR response of Bacillus subtilis.
J Bacteriol: 2010, 192(18);4680-93
[PubMed:20639339] [WorldCat.org] [DOI] (I p)

Anna-Barbara Hachmann, Esther R Angert, John D Helmann
Genetic analysis of factors affecting susceptibility of Bacillus subtilis to daptomycin.
Antimicrob Agents Chemother: 2009, 53(4);1598-609
[PubMed:19164152] [WorldCat.org] [DOI] (I p)

Sina Jordan, Eva Rietkötter, Mark A Strauch, Falk Kalamorz, Bronwyn G Butcher, John D Helmann, Thorsten Mascher
LiaRS-dependent gene expression is embedded in transition state regulation in Bacillus subtilis.
Microbiology (Reading): 2007, 153(Pt 8);2530-2540
[PubMed:17660417] [WorldCat.org] [DOI] (P p)

Sina Jordan, Anja Junker, John D Helmann, Thorsten Mascher
Regulation of LiaRS-dependent gene expression in bacillus subtilis: identification of inhibitor proteins, regulator binding sites, and target genes of a conserved cell envelope stress-sensing two-component system.
J Bacteriol: 2006, 188(14);5153-66
[PubMed:16816187] [WorldCat.org] [DOI] (P p)

Thorsten Mascher, Sara L Zimmer, Terry-Ann Smith, John D Helmann
Antibiotic-inducible promoter regulated by the cell envelope stress-sensing two-component system LiaRS of Bacillus subtilis.
Antimicrob Agents Chemother: 2004, 48(8);2888-96
[PubMed:15273097] [WorldCat.org] [DOI] (P p)

Mélanie A Hamon, Nicola R Stanley, Robert A Britton, Alan D Grossman, Beth A Lazazzera
Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis.
Mol Microbiol: 2004, 52(3);847-60
[PubMed:15101989] [WorldCat.org] [DOI] (P p)