Difference between revisions of "LevE"
| Line 93: | Line 93: | ||
* '''Operon:''' ''[[levD]]-[[levE]]-[[levF]]-[[levG]]-[[sacC]]'' | * '''Operon:''' ''[[levD]]-[[levE]]-[[levF]]-[[levG]]-[[sacC]]'' | ||
| − | * '''Sigma factor:''' [[SigL]] | + | * '''[[Sigma factor]]:''' [[SigL]] |
* '''Regulation:''' repressed by glucose ([[CcpA]]) , carbon catabolite repression, induction by fructose | * '''Regulation:''' repressed by glucose ([[CcpA]]) , carbon catabolite repression, induction by fructose | ||
Revision as of 09:18, 8 April 2009
- Description: trigger enzyme: fructose-specific phosphotransferase system, EIIB component
| Gene name | levE |
| Synonyms | sacL |
| Essential | no |
| Product | trigger enzyme: fructose-specific phosphotransferase system, EIIB component
fructose-specific enzyme IIB (EC 2.7.1.69) |
| Function | fructose uptake and phosphorylation, control of LevR activity |
| MW, pI | 17 kDa, 9.444 |
| Gene length, protein length | 486 bp, 162 aa |
| Immediate neighbours | levF, levD |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: PTS permease, mannose permease (Man) family PubMed
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry:
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation: repressed by glucose (CcpA) , carbon catabolite repression, induction by fructose
- Regulatory mechanism: CcpA: transcription repression, catabolite repression: transcription repression by CcpA, transcription activator LevR is less active in the presence of glucose; induction: transcription activation by LevR
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology 145: 3419-3429 PubMed
- Martin-Verstraete, I., Débarbouillé, M., Klier, A., and Rapoport, G. (1990) Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon. J Mol Biol 214: 657-671. PubMed
- Martin-Verstraete, I., Charrier, V., Stülke, J., Galinier, A., Erni, B., Rapoport, G., & Deutscher, J. (1998) Antagonistic effects of dual PTS catalyzed phosphorylation on the Bacillus subtilis transcriptional activator LevR. Mol. Microbiol. 28: 293-303. PubMed
- Schauder S, Nunn RS, Lanz R, Erni B, Schirmer T. 1998. Crystal structure of the IIB subunit of a fructose permease (IIBLev) from Bacillus subtilis. J. Mol. Biol. 276:591-602. PubMed
- Seip, S., R. Lanz, R. Gutknecht, K. Flükiger, and B. Erni. 1997. The fructose transporter of Bacillus subtilis encoded by the lev operon. Backbone assignment and secondary structure of the IIBLev subunit. Eur. J. Biochem. 243: 306-314. PubMed
- Charrier V, Deutscher J, Martin-Verstraete I (1997b) Protein phosphorylation chain of a Bacillus subtilis fructose-specific phosphotransferase system and its participation in regulation of the expression of the lev operon. Biochemistry 36:1163-1172. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
