Difference between revisions of "LeuD"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):''' contains an iron-sulfur cluster
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* '''[[Cofactors]]:''' contains an iron-sulfur cluster
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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=== Additional information===
 
=== Additional information===
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* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
  
:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
 
 
=Expression and regulation=
 
=Expression and regulation=
  
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=leuD_2888940_2889539_-1 leuD] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=leuD_2888940_2889539_-1 leuD] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1577690 PubMed]
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* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1577690 PubMed]
  
* '''Regulation:''' for a complete overview on the regulation of the ilv operon, see [http://www.ncbi.nlm.nih.gov/pubmed/20935095 Brinsmade ''et al''.]
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* '''Regulation:'''  
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** for a complete overview on the regulation of the ilv operon, see [http://www.ncbi.nlm.nih.gov/pubmed/20935095 Brinsmade ''et al''.]
 
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]  
 
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]  
 
** expression is stimulated in the presence of glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/12193635 PubMed]
 
** expression is stimulated in the presence of glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/12193635 PubMed]
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=References=
 
=References=
  
<pubmed>15060025,12193635,19258532,18697947,8289305,18641142,11948165,15547269,12618455,15547269,12618455,12107147, 20935095</pubmed>
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<pubmed>15060025,12193635,19258532,18697947,8289305,18641142,11948165,15547269,12618455,15547269,12618455,12107147, 20935095 24163341</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:37, 22 December 2013

  • Description: 3-isopropylmalate dehydratase (small subunit)

Gene name leuD
Synonyms
Essential no
Product 3-isopropylmalate dehydratase (small subunit)
Function biosynthesis of leucine
Gene expression levels in SubtiExpress: leuD
Metabolic function and regulation of this protein in SubtiPathways:
Ile, Leu, Val
MW, pI 22 kDa, 4.582
Gene length, protein length 597 bp, 199 aa
Immediate neighbours ysoA, leuC
Sequences Protein DNA DNA_with_flanks
Genetic context
LeuD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LeuD expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

CcpA regulon, CodY regulon, FsrA regulon, T-box, TnrA regulon

The gene

Basic information

  • Locus tag: BSU28250

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmaleate + H2O (according to Swiss-Prot)
  • Protein family: LeuD type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 2PKP (from Methanocaldococcus jannaschii dsm 2661, 38% identity, 53% similarity)
  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation:
    • for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
    • repressed by casamino acids PubMed
    • expression is stimulated in the presence of glucose PubMed
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
    • less expressed under conditions of extreme iron limitation (FsrA) PubMed
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References