Difference between revisions of "LeuB"
(→Expression and regulation) |
|||
| Line 96: | Line 96: | ||
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1577690 PubMed] | * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1577690 PubMed] | ||
| − | * '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | + | * '''Regulation:''' |
| + | ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | ||
| + | ** expression is stimulated in the presence of glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/12193635 PubMed] | ||
** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15547269 PubMed] | ** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15547269 PubMed] | ||
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ||
| − | * '''Regulatory mechanism:''' [[ | + | * '''Regulatory mechanism:''' |
| + | ** [[CcpA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/12193635 PubMed] | ||
| + | ** [[T-box]]: tRNA-controlled [[RNA switch]] that mediates termination/antitermination | ||
** [[TnrA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15547269 PubMed] | ** [[TnrA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15547269 PubMed] | ||
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ||
Revision as of 20:21, 13 December 2009
- Description: 3-isopropylmalate dehydrogenase
| Gene name | leuB |
| Synonyms | leuC |
| Essential | no |
| Product | 3-isopropylmalate dehydrogenase |
| Function | biosynthesis of leucine |
| Metabolic function and regulation of this protein in SubtiPathways: Ile, Leu, Val, Coenzyme A | |
| MW, pI | 39 kDa, 4.744 |
| Gene length, protein length | 1095 bp, 365 aa |
| Immediate neighbours | leuC, leuA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU28270
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH (according to Swiss-Prot)
- Protein family: LeuB type 1 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 1XAD (Thermus thermophilus)
- UniProt: P05645
- KEGG entry: [3]
- E.C. number: 1.1.1.85
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
