LeuA

• Description: 2-isopropylmalate synthase
  
  

Gene name	leuA
Synonyms
Essential	no
Product	2-isopropylmalate synthase
Function	biosynthesis of leucine
Gene expression levels in SubtiExpress: leuA
Metabolic function and regulation of this protein in SubtiPathways: Ile, Leu, Val, Coenzyme A
MW, pI	56 kDa, 5.657
Gene length, protein length	1554 bp, 518 aa
Immediate neighbours	leuB, ilvC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

CcpA regulon, CodY regulon, T-box, TnrA regulon

The gene

Basic information

• Locus tag: BSU28280

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acetyl-CoA + 3-methyl-2-oxobutanoate + H₂O = (2S)-2-isopropylmalate + CoA (according to Swiss-Prot)

• Protein family: LeuA type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot), membrane PubMed

Database entries

• Structure: 3EEG (from Cytophaga hutchinsonii atcc 33406, 53% identity, 68% similarity)

• UniProt: P94565

• KEGG entry: [3]

• E.C. number: 2.3.3.13

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD PubMed

• Expression browser: leuA PubMed

• Sigma factor: SigA PubMed

• Regulation: for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
  • repressed by casamino acids PubMed
  • expression is stimulated in the presence of glucose PubMed
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY, T-box) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • CcpA: transcription activation PubMed
  • T-box: tRNA-controlled RNA switch that mediates termination/antitermination
  • TnrA: transcription repression PubMed

• Additional information:
  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • An antisense RNA is predicted forleuA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: