Difference between revisions of "LeuA"

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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + 3-methyl-2-oxobutanoate + H<sub>2</sub>O = (2S)-2-isopropylmalate + CoA (according to Swiss-Prot)
  
 
* '''Protein family:''' LeuA type 1 subfamily (according to Swiss-Prot)
 
* '''Protein family:''' LeuA type 1 subfamily (according to Swiss-Prot)

Revision as of 11:35, 24 May 2009

  • Description: 2-isopropylmalate synthase

Gene name leuA
Synonyms
Essential no
Product 2-isopropylmalate synthase
Function biosynthesis of leucine
MW, pI 56 kDa, 5.657
Gene length, protein length 1554 bp, 518 aa
Immediate neighbours leuB, ilvC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LeuA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA (according to Swiss-Prot)
  • Protein family: LeuA type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot), membrane PubMed

Database entries

  • Structure:

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation: repressed by casamino acids PubMed , expressed in the absence of branched-chain amino acids (BCAA), expression is stimulated in the presence of glucose PubMed, repressed by CodY PubMed
  • Regulatory mechanism: CodY: transcription repression PubMed, glucose regulation: CcpA PubMed, repression by BCAA: tRNA-controlled RNA switch (T-box) that mediates termination/antitermination
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
  2. Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 PubMed
  3. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
  4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed