Difference between revisions of "LacR"

From SubtiWiki
Jump to: navigation, search
Line 14: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of galactan utilization  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of galactan utilization  
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34170 lacR]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34170 lacR]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 5.396   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 5.396   
Line 22: Line 22:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cycB]]'', ''[[lutR]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cycB]]'', ''[[lutR]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15422&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34170 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34170 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34170 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:lacR_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:lacR_context.gif]]

Revision as of 13:53, 13 May 2013

  • Description: transcriptional repressor of the galactan utilization operon

Gene name lacR
Synonyms yvfJ
Essential no
Product transcriptional repressor (LacI family)
Function regulation of galactan utilization
Gene expression levels in SubtiExpress: lacR
MW, pI 37 kDa, 5.396
Gene length, protein length 990 bp, 330 aa
Immediate neighbours cycB, lutR
Sequences Protein DNA Advanced_DNA
Genetic context
LacR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LacR expression.png
























Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control

This gene is a member of the following regulons

The LacR regulon:

The gene

Basic information

  • Locus tag: BSU34170

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP815 (spc) available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

R A Daniel, J Haiech, F Denizot, J Errington
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol: 1997, 179(17);5636-8
[PubMed:9287030] [WorldCat.org] [DOI] (P p)

J Errington, C H Vogt
Isolation and characterization of mutations in the gene encoding an endogenous Bacillus subtilis beta-galactosidase and its regulator.
J Bacteriol: 1990, 172(1);488-90
[PubMed:2104611] [WorldCat.org] [DOI] (P p)