Difference between revisions of "KtrA"

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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
** [[KtrA]]-[[KtrB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed]
+
** [[KtrA]]-[[KtrB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed], composed of an octameric [[KtrA]] ring and two [[KtrB]] dimers {{PubMed|23598340}}
  
 
* '''[[Localization]]:''' peripheral membrane protein [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed]
 
* '''[[Localization]]:''' peripheral membrane protein [http://www.ncbi.nlm.nih.gov/sites/entrez/12562800 PubMed]
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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1LSU 1LSU] (complex with NADH)[http://www.proteopedia.org/wiki/index.php/2hmw 2HMW] ( complex with ATP)
+
* '''Structure:'''  
 +
** [http://www.rcsb.org/pdb/explore.do?structureId=1LSU 1LSU] (complex with NADH)
 +
** [http://www.proteopedia.org/wiki/index.php/2hmw 2HMW] ( complex with ATP)
 +
** [http://www.pdb.org/pdb/explore/explore.do?structureId=4J7C 4J7C] (the [[KtrA]]-[[KtrB]] complex) {{PubMed|23598340}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O32080 O32080]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O32080 O32080]
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ktrA_3188414_3189082_1 ktrA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ktrA_3188414_3189082_1 ktrA] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
+
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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=References=
 
=References=
  
<pubmed>12562800,15096624, 20511502, 23086297</pubmed>
+
<pubmed>12562800,15096624, 20511502, 23086297 23598340 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:06, 22 April 2013

  • Description: high affinity potassium transporter KtrA-KtrB, peripheric membrane component (proton symport)

Gene name ktrA
Synonyms yuaA
Essential no
Product high affinity potassium transporter KtrA-KtrB,
peripheric membrane component (proton symport)
Function potassium uptake
Gene expression levels in SubtiExpress: ktrA
Interactions involving this protein in SubtInteract: KtrA
Metabolic function and regulation of this protein in SubtiPathways:
Metal ion homeostasis, Stress
MW, pI 24 kDa, 5.981
Gene length, protein length 666 bp, 222 aa
Immediate neighbours bslA, ktrB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YuaA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
KtrA expression.png















Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

YdaO riboswitch

The gene

Basic information

  • Locus tag: BSU31090

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): KtrC

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

Your additional remarks

References

Ricardo S Vieira-Pires, Andras Szollosi, João H Morais-Cabral
The structure of the KtrAB potassium transporter.
Nature: 2013, 496(7445);323-8
[PubMed:23598340] [WorldCat.org] [DOI] (I p)

Peter Y Watson, Martha J Fedor
The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis.
Nat Chem Biol: 2012, 8(12);963-5
[PubMed:23086297] [WorldCat.org] [DOI] (I p)

Kirsten F Block, Ming C Hammond, Ronald R Breaker
Evidence for widespread gene control function by the ydaO riboswitch candidate.
J Bacteriol: 2010, 192(15);3983-9
[PubMed:20511502] [WorldCat.org] [DOI] (I p)

Jeffrey E Barrick, Keith A Corbino, Wade C Winkler, Ali Nahvi, Maumita Mandal, Jennifer Collins, Mark Lee, Adam Roth, Narasimhan Sudarsan, Inbal Jona, J Kenneth Wickiser, Ronald R Breaker
New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control.
Proc Natl Acad Sci U S A: 2004, 101(17);6421-6
[PubMed:15096624] [WorldCat.org] [DOI] (P p)

Gudrun Holtmann, Evert P Bakker, Nobuyuki Uozumi, Erhard Bremer
KtrAB and KtrCD: two K+ uptake systems in Bacillus subtilis and their role in adaptation to hypertonicity.
J Bacteriol: 2003, 185(4);1289-98
[PubMed:12562800] [WorldCat.org] [DOI] (P p)